Figure 2From: Critical aspects of using bacterial cell viability assays with the fluorophores SYTO9 and propidium iodide Bleaching of SYTO9 over time. Different amounts of live or dead cells of S. aureus (S.a.) and P. aeruginosa (P.a.) were stained with SYTO9, respectively. After 15Â min incubation fluorescence intensity at 528Â nm was automatically measured every 5Â minutes with the microplate reader. Starting RFU value was set to 100% which was used to normalize other values.Back to article page