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Figure 2 | BMC Microbiology

Figure 2

From: Critical aspects of using bacterial cell viability assays with the fluorophores SYTO9 and propidium iodide

Figure 2

Bleaching of SYTO9 over time. Different amounts of live or dead cells of S. aureus (S.a.) and P. aeruginosa (P.a.) were stained with SYTO9, respectively. After 15 min incubation fluorescence intensity at 528 nm was automatically measured every 5 minutes with the microplate reader. Starting RFU value was set to 100% which was used to normalize other values.

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