ΔMomyb1 mutants are more insensitive to osmotic stresses. (A) Wild type Guy11, ΔMomyb1 mutants and complemented transformant were incubated on CM plates containing various concentrations of NaCl, KCl or sorbitol at 28°C for 7 days. (B) The growth rate was determined 7 days after incubation at 28°C by plotting the percentage of colonies in the presence of various concentrations of NaCl, KCl or sorbitol against regular CM. (C) qRT-PCR analysis the transcription of four components of the Hog1 pathway in M. oryzae. Asterisks were indicated significant differences at P < 0.01.