Effect of MoMYB1 deletion on pathogenicity. (A) Detached rice leaf assay. Intact rice leaves were inoculated by mycelium plugs from wild type Guy11, ΔMomyb1 mutant and complemented transformant ΔMomyb1/MoMYB1. Photographs were taken at 7 days after inoculation. (B) Rice root infection assay. Lesions were examined at 9 days post-inoculation. CK: inoculated with agar plugs without hyphae. (C) Observation of the invasive hyphal growth inside the rice root inoculated with the Guy11 and ΔMomyb1 strain expression a GFP protein, respectively. White arrows point to invasive hyphae. Bar = 50 μm. (D) Close view the invasive hyphae in plant cells. White arrows point to invasive hyphae. Bar = 10 μm.