Figure 7

FaTBCA is required for full virulence and DON biosynthesis in F. asiaticum . A. Flowering wheat heads were point inoculated with a conidial suspension at 10⁵ conidia ml⁻¹ of the wild-type GJ33, ΔFaTBCA-3 and ΔFaTBCA-3C, and infected wheat heads were photographed 15 days after inoculation. B. Tomatoes were inoculated with a conidial suspension at 10⁵ conidia ml⁻¹ of each strain and infected fruits were photographed 3 days after inoculation. C. The amount of DON (per mg fungal ergosterol) produced by each strain in infected wheat kernels was determined after 20 days of inoculation. Line bars in each column denote standard errors of three experiments. Values on the black bars followed by the same letter are not significantly different according to a Fisher’s least significant difference (LSD) test at P = 0.05. D. Relative expression of DON biosynthetic TRI genes in each strain and bars denote standard errors from three experiments. Values on the black bars followed by the same letter for each gene are not significantly different according to a Fisher’s least significant difference (LSD) test at P = 0.05.