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Table 2 List of bacterial strains

From: Hyperphosphorylation of DegU cancels CcpA-dependent catabolite repression of rocG in Bacillus subtilis

Strain

Genotype

Source or reference

B. subtilis

  

168

trpC2

Laboratory stock

1A95

trpC2 degU32

[14]

WTF28

degU::cat

[8].

BFS3227

trpC2 sivA::pMutin2

NIG, Japan

ΔrocR (FW15)

pheA1 sfp0 trpC2 rocR::kan

[55]

FU402

trpC2 ccpA::neo

[57]

TM013

trpC2 rocG::cat

This study

TM014

trpC2 degU32 rocG::cat

This study

TM015

trpC2 degU32::cat

This study

TM016

trpC2 degU32 rocR::kan

This study

TM017

trpC2 sivA::pMutin2

This study

TM018

trpC2 degU32 sivA::pMutin2

This study

TM023

trpC2 ccpA::neo

This study

TM024

trpC2 degU32 ccpA::neo

This study

MY02

trpC2 lctE::spc

This study

KI004

trpC2 degU32 lctE::spc

This study

E. coli

  

DH5α

supE44 ΔlacU169 hsdR17 recA1 endA1 gyrA1 gyrA96 thi-1 relA1

Laboratory stock

Plasmid

  

pGP958

rocG::cat

[54]