Figure 8

Northern blotting analyses of transcripts containing ptsG , licC , and dra. RNAs were prepared from 168 (lane 1), TM023 (ccpA::neo, lane 2), 1A95 (degU32, lane 3), TM024 (degU32 ccpA::neo, lane 4), and TM015 (degU32::cat, lane 5) cells for northern blotting analyses using probes for ptsG (A), licC (B), and dra (C) transcripts under higher-stringency conditions. Experiments were repeated independently at least three times with similar results and representative data are shown; rRNAs (23S and 16S) are shown on the membranes as loading controls. Genetic schemas of respective loci are shown below.