Figure 5

Intracellular levels of central carbon metabolism intermediates. Cells of 168 (closed squares) and 1A95 (degU32, open squares) strains were grown in the soytone–glucose medium and intracellular metabolites were extracted at the indicated time points after inoculation for CE–MS analyses. Experiments were repeated independently for three times, and the mean values are shown with standard deviation. Intermediates are abbreviated as follows: glucose 6-phosphate, G6P; fructose-1,6-bis-phosphate, FBP; 3-phosphoglyceric acid, 3PG; phosphoenolpyruvate, PEP; pyruvate, PYR; 6-phosphogluconate, 6PG; ribulose 5-phosphate, Ru5P; and sedoheptulose 7-phosphate, S7P.