Figure 4

Northern blotting analyses of rocG transcripts in the presence and absence of ccpA. RNAs were prepared from 168 (lane 1), TM023 (ccpA::neo, lane 2), 1A95 (degU32, lane 3), and TM024 (degU32 ccpA::neo, lane 4) cells for northern blotting analyses using a probe for rocG transcripts under high-stringency conditions. Experiments were repeated independently at least three times with similar results, and representative data are shown. At the bottom, rRNAs (23S and 16S) were visualized as loading controls using methylene blue staining.