Figure 3

The promoter and terminator regions of sivA. (A) Primer extension analyses of the sivA promoter region; RNAs were prepared from 168 (lane 1) and 1A95 (degU32, lane 2) cells, and were reverse transcribed to generate cDNAs corresponding to the 5′-terminus of sivA transcripts. Lanes G, A, T, and C contained dideoxy sequencing ladders. The partial nucleotide sequence of the coding strand is shown on the right side, where the putative −10 region and the transcription start site (+1) are shown in enlarged uppercase characters. (B) Schematic presentation of the sivA promoter region. The nucleotide sequence of the upper strand of the promoter region is shown, and putative −35 and −10 regions, the transcription start site (+1), and the beginning of the sivA open reading frame are shown with enlarged uppercase characters. (C) Nucleotide sequence of the intergenic region between sivA and rocG. The C-terminal end of sivA, the Rho-independent terminator, SigL-dependent rocG promoter (+35 and −10 regions), its transcription start site (+1), the cre site, and the N-terminal end of rocG are indicated.