Lactobacillus reuteri I5007 modulates tight junction protein expression in IPEC-J2 cells with LPS stimulation and in newborn piglets under normal conditions

Table 1 Primers used for real-time PCR

Genes	Primers	Sequences (5'-3')	Size (bp)	Tm (°C)
TNF-α	Forward	ATTCAGGGATGTGTGGCCTG	120	62
	Reverse	CCAGATGTCCCAGGTTGCAT
IL-6	Forward	TGGATAAGCTGCAGTCACAG	109	54
	Reverse	ATTATCCGAATGGCCCTCAG
Claudin-1	Forward	GCAGCAGCTTCTTGCTTCTC	664	58
	Reverse	CTGGCATTGACTGGGGTCAT
Occludin	Forward	ATCAACAAAGGCAACTCT	157	50
	Reverse	GCAGCAGCCATGTACTCT
ZO-1	Forward	GAGTTTGATAGTGGCGTT	298	50
	Reverse	GTGGGAGGATGCTGTTGT
β-actin	Forward	TGCGGGACATCAAGGAGAAG	216	60
	Reverse	AGTTGAAGGTGGTCTCGTGG

ISSN: 1471-2180