Levels of TJ proteins in LPS-activated IPEC-J2 cells at different time points. Proteins were isolated from IPEC-J2 cells and the expression of TJ proteins was assessed using immunoblotting. There was no significant difference in claudin-1, occludin and ZO-1 expression among the different treatments at 1 h (A). LPS produced decreases in all three TJ proteins at 4 (B), 8 (C), 12 (D) and 24 (E) h. Although L. reuteri I5007 (LR) and its culture supernatant (CS) had no effect on the levels of TJ proteins in the absence of LPS, they inhibited LPS-induced levels of TJ proteins. Results are represented as mean ± SEM, n = 3. *P < 0.05 vs. control cells, and #P < 0.05 vs. cells in the LPS group.