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Figure 6 | BMC Microbiology

Figure 6

From: A tyrosine phosphorylation switch controls the interaction between the transmembrane modulator protein Wzd and the tyrosine kinase Wze of Lactobacillus rhamnosus

Figure 6

Detection of tyrosine phosphorylated GST-Wze released in the wash fractions. GST-Wze in wash fractions in the presence of 10 mM ATP (1–3 and 7–9) and absence of ATP (4–6 and 10–12) from interaction of GST-Wze with resin-bound His6-Wzd expressed by E. coli BL21(DE3): (A) Coomassie stained 12% SDS-PAGE gel and (B) Western blot probed with mouse monoclonal anti-phosphotyrosine antibody. GST-Wze in wash fractions in the presence of 10 mM ATP (13–15 and 19–21) and absence of ATP (16–18 and 22–24) from interaction of GST-Wze with His6-Wzd expressed by E. coli C41: (C) Coomassie stained 12% SDS-PAGE gel and (D) Western blot probed with mouse monoclonal anti-phosphotyrosine antibody.

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BMC Microbiology

ISSN: 1471-2180

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