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Figure 3 | BMC Microbiology

Figure 3

From: A tyrosine phosphorylation switch controls the interaction between the transmembrane modulator protein Wzd and the tyrosine kinase Wze of Lactobacillus rhamnosus

Figure 3

Western immunoblot of the phosphorylation state of His 6 -Wzd and GST-Wze proteins expressed in E. coli. Tyrosine phosphorylation of His6-Wzd and GST-Wze was detected by using Western immunoblotting with a mouse monoclonal antibody. Coomassie stained 12% SDS-PAGE gel of His6-Wzd expressed in E. coli BL21(DE3) (A) and C41(DE3) (B): incubated with 10 mM ATP (Lane 1) and without ATP (Lane 2). (C) Detection of tyrosine phosphorylated His6-Wzd expressed in E. coli BL21(DE3) and incubated with 10 mM ATP (Lane 1) and without ATP (Lane 2). (D) Absence of detection of tyrosine phosphorylated His6-Wzd expressed in E. coli C41(DE3) and incubated with 10 mM ATP (Lane 1) and without ATP (Lane 2). (E) Detection of tyrosine phosphorylated GST-Wze expressed in E. coli C41(DE3) incubated with 10 mM ATP (Lane 1) and without ATP (Lane 2).

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BMC Microbiology

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