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Figure 1 | BMC Microbiology

Figure 1

From: A tyrosine phosphorylation switch controls the interaction between the transmembrane modulator protein Wzd and the tyrosine kinase Wze of Lactobacillus rhamnosus

Figure 1

Immunoblot showing tyrosine phosphorylation of wild-type Wzd, ΔWzd and Wze proteins expressed in L. lactis subsp. cremoris MG1363 . Detection of tyrosine phosphorylation of Wze (27 kDa) along with wild type Wzd (33 kDa) or nine separate tyrosine mutated Wzd versions (33 kDa) expressed in L. lactis subsp. cremoris MG1363 with plasmids pDY33FEB (lane 1), pDY44FEB (lane 2), pDY77FEB (lane 3), pDY110FEB (lane 4), pDY114FEB (lane 5), pDY124FEB (lane 6), pDY134FEB (lane 7), pDY266FEB (lane 8), pDY141FEB (lane 9), pDWTEB (lane 10). Cell lysates of L. lactis subsp. cremoris MG1363 transformants were separated by 12% SDS-PAGE and tyrosine phosphorylated proteins were probed with mouse monoclonal anti-phosphotyrosine antibody.

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BMC Microbiology

ISSN: 1471-2180

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