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Table 4 Proteolytic and ureolytic activities of hyper-ammonia-producing bacterial isolates* obtained from Nellore steers

From: Biochemical and genetic diversity of carbohydrate-fermenting and obligate amino acid-fermenting hyper-ammonia-producing bacteria from Nellore steers fed tropical forages and supplemented with casein

Isolate ID**

Proteolytic activity in trypticase 1

Proteolytic activity in Casein 1

Ureolytic activity in trypticase + urea 2

C11

0.12 ± 0.01

0.86 ± 0.06

1.74 ± 0.01

C33

0.01 ± 0.01

1.18 ± 0.04

0.80 ± 0.01

C34

0.41 ± 0.01

10.87 ± 0.08

2.37 ± 0.05

C37

0.07 ± 0.01

0.40 ± 0.03

6.03 ± 0.15

C47

0.16 ± 0.01

0.89 ± 0.08

12.07 ± 0.06

C48

1.19 ± 0.03

4.30 ± 0.16

0.78 ± 0.01

C51

0.09 ± 0.01

0.18 ± 0.01

1.96 ± 0.03

C54

1.61 ± 0.01

5.17 ± 0.26

3.46 ± 0.04

C89

0.13 ± 0.01

2.52 ± 0.02

6.01 ± 0.10

C114

0.17 ± 0.01

0.66 ± 0.01

1.50 ± 0.03

C116

0.14 ± 0.01

0.83 ± 0.07

3.10 ± 0.01

C117

1.25 ± 0.01

31.26 ± 1.76

15.55 ± 0.39

C118

0.13 ± 0.01

0.91 ± 0.01

4.81 ± 0.03

C122

0.70 ± 0.01

1.36 ± 0.09

2.33 ± 0.02

R15

0.11 ± 0.01

0.38 ± 0.01

2.06 ± 0.08

R21

0.12 ± 0.01

0.81 ± 0.04

1.05 ± 0.01

R23

0.09 ± 0.01

4.02 ± 0.16

1.81 ± 0.03

R34

0.34 ± 0.02

0.91 ± 0.08

1.28 ± 0.01

R36

0.91 ± 0.01

1.55 ± 0.09

3.77 ± 0.08

R40

2.46 ± 0.02

9.48 ± 0.37

7.44 ± 0.04

R50

0.78 ± 0.03

1.65 ± 0.17

2.67 ± 0.03

R51

0.85 ± 0.01

0.87 ± 0.04

1.41 ± 0.02

R60

0.08 ± 0.01

0.61 ± 0.06

1.36 ± 0.02

R61

0.10 ± 0.01

0.42 ± 0.04

1.33 ± 0.02

R63

0.13 ± 0.01

0.46 ± 0.07

1.11 ± 0.01

R90

0.22 ± 0.01

0.79 ± 0.07

2.41 ± 0.03

R91

0.22 ± 0.01

1.38 ± 0.38

2.07 ± 0.04

R96

0.19 ± 0.01

0.68 ± 0.09

1.55 ± 0.03

R97

0.05 ± 0.01

1.17 ± 0.13

4.00 ± 0.06

R107

0.12 ± 0.01

0.99 ± 0.31

10.37 ± 0.41

  1. *Cultures were incubated for 48 h at 39°C in anaerobic mineral medium with limited nitrogen before the enzymatic activities were determined.
  2. **C, isolates from animals not supplemented with casein; R, isolates obtained from animals that received ruminal infusion of casein.
  3. 1U μg protein−1.
  4. 2μmol NH3 mg protein−1 min−1.
  5. Values are given as mean ± standard deviation of the mean.