Biochemical and genetic diversity of carbohydrate-fermenting and obligate amino acid-fermenting hyper-ammonia-producing bacteria from Nellore steers fed tropical forages and supplemented with casein

Table 3 **Utilization of different carbon sources by hyper-ammonia-producing bacterial isolates* obtained from Nellore steers**

Isolate ID**	Trypticase	Cellobiose	Glucose	Maltose	Xylose	Casein	Trypticase + urea
C11	+ + +	-	+ +	+	-	-	+
C33	+ + +	+	++	-	-	-	+
C34	+ + +	+	-	-	+	-	+
C37	+ + +	-	+ +	+	-	+	+
C47	+ + +	-	+	-	-	-	+ +
C48	+ + +	-	+ + +	+ + +	-	-	+
C51	+ + +	+	+ + +	+ + +	+	++	+ +
C54	+ + +	+ +	+	+ +	-	-	+ +
C89	+ + +	+	+ +	+ +	+	+	+ +
C114	+ +	+ +	+ +	+ +	+	+	+
C116	+ +	-	+	+	-	-	+ +
C117	+ + +	+	+	+	+	+	+ +
C118	+ + +	+ +	+	+	-	-	+ +
C122	+ + +	+	+ + +	+ +	-	+	+ +
R15	+ + +	-	+ + +	+ +	+	+	+ +
R21	+ + +	-	-	-	-	+	+
R23	+ + +	-	+	-	-	+	+ +
R34	+ + +	+	+	+ +	-	-	+
R36	+ + +	-	+ +	-	+	+	+
R40	+ + +	-	-	-	-	-	+
R50	+ + +	-	-	-	-	-	+
R51	+ + +	-	-	+	-	-	+
R60	+ +	+	+	-	-	+	+ +
R61	+ +	+	+	+	-	+	+ +
R63	+ +	+	+	+	-	+	+ +
R90	+ + +	+	+ +	+	+	+	+ +
R91	+ + +	-	+	+	-	+	+
R96	+ +	+	+ +	+	-	+	+ +
R97	+ + +	-	+	-	-	-	+
R107	+ + +	-	+	-	-	+	+ + +

*Growth using carbohydrates (2.0 g l⁻¹) and trypticase (15 g l⁻¹) was determined following incubation for 24 h at 39°C. Utilization of casein (4.0 g l⁻¹) and trypticase (7.5 g l⁻¹) plus urea (2.0 g l⁻¹) was determined following incubation of the isolates at 39°C for 48 h.
**C, isolates from animals not supplemented with casein; R, isolates obtained from animals that received ruminal infusion of casein.
(−) non-growth (OD₆₀₀ < 0.150); (+) change in OD₆₀₀ 0.150–0.300; (+ +) change in OD₆₀₀ 0.300–0.450; (+ + +) OD₆₀₀ > 0.450.

ISSN: 1471-2180