Biochemical and genetic diversity of carbohydrate-fermenting and obligate amino acid-fermenting hyper-ammonia-producing bacteria from Nellore steers fed tropical forages and supplemented with casein

Table 2 Fermentation products (mmol l ⁻¹ **) of hyper-ammonia-producing bacterial isolates* obtained from Nellore steers**

Concentration of fermentation end-products	Isolate ID	Total VFA	A	P	B	Ib	F	S	Iv
		(mmol l ⁻¹ **)				(%)
High	C48	79.9 ± 2.35a	47.8	8.1	7.8	5.3	4.37	4.8	21.5
	C51	76.6 ± 1.74a	54.2	7.1	7.3	5.1	1.57	1.5	23.0
	C33	73.3 ± 3.16a	55.4	7.9	5.8	5.9	2.37	nd	22.3
	R34	71.0 ± 1.30a	37.4	3.7	5.9	5.3	1.78	1.7	44.0
	C11	68.4 ± 4.84a	69.1	11.8	6.2	7.2	1.32	nd	4.2
	R36	66.7 ± 3.14a	50.3	11.3	5.1	5.1	1.48	nd	26.5
	C47	64.4 ± 1.94a	49.2	8.4	7.7	5.8	1.95	nd	26.7
	C37	62.5 ± 0.99a	65.9	13.9	6.8	6.5	1.69	nd	5.0
	R40	60.6 ± 5.15a	37.8	10.2	5.5	4.0	9.48	nd	32.9
	R90	60.2 ± 2.34a	64.9	9.7	5.4	9.7	3.63	1.5	4.9
	R15	59.6 ± 2.90a	67.4	10.1	6.9	8.2	1.94	0.5	4.7
	R23	53.7 ± 3.47a	61.8	12.0	8.3	9.5	5.14	nd	3.0
Medium	C89	49.2 ± 1.69b	57.1	9.5	7.3	6.0	14.9	2.5	2.4
	C34	45.5 ± 3.25b	63.3	14.6	7.2	7.2	3.00	nd	4.5
	C122	45.0 ± 2.42b	57.9	10.4	7.3	5.6	13.44	2.9	2.2
	R50	44.2 ± 2.09b	66.8	10.1	7.3	7.8	2.89	nd	4.9
	R61	44.0 ± 3.20b	39.0	7.3	6.6	4.4	nd	nd	42.5
	C114	43.6 ± 1.05b	51.4	10.4	6.5	7.8	21.72	nd	2.0
	C118	41.6 ± 1.21b	55.7	10.1	7.4	7.9	12.63	3.2	2.6
	R51	39.0 ± 3.43b	50.1	16.9	9.0	6.8	13.43	1.6	1.9
	C54	38.3 ± 1.88b	51.5	16.5	8.8	8.3	12.86	nd	1.8
	C117	34.5 ± 3.92b	49.4	15.0	2.6	13.6	8.69	6.3	4.1
Low	R21	30.6 ± 6.17c	71.5	9.0	8.7	5.9	nd	nd	4.7
	R60	30.3 ± 2.56c	66.0	8.4	13.5	6.2	nd	nd	5.8
	R91	25.0 ± 1.05c	20.2	10.2	3.5	56.2	8.02	nd	1.6
	R63	22.9 ± 0.61c	64.7	11.6	10.3	7.3	nd	nd	5.9
	R107	21.5 ± 2.11c	58.8	15.4	3.6	13.5	6.59	nd	1.9
	C116	19.3 ± 4.17c	50.0	17.2	nd	15.4	10.61	6.6	nd
	R96	16.9 ± 3.34c	52.2	17.3	4.5	11.6	11.13	nd	3.0
	R97	12.2 ± 5.97c	47.8	25.7	nd	7.3	19.05	nd	nd

A = acetate; P = propionate; B = butyrate; Ib = isobutyrate; F = formate; S = succinate; Iv = isovalerate; nd = not detected.
Values are provided as the mean ± standard deviation of the mean.
*The isolates were grown in anaerobic mineral medium supplemented with 15 g l⁻¹ trypticase for 24 h at 39°C.
**Averages of total volatile fatty acids followed by different letters in the same column differ at 5% probability by the Scott-Knott test.

ISSN: 1471-2180