Figure 1From: Biochemical and genetic diversity of carbohydrate-fermenting and obligate amino acid-fermenting hyper-ammonia-producing bacteria from Nellore steers fed tropical forages and supplemented with casein Electrophoretic profile of 16S rRNA gene sequences of rumen bacteria obtained by denaturing gradient gel electrophoresis. The animals were fed hay, with or without casein supplementation. The profiles were obtained after nested-PCR amplification of genomic DNA extracted from ruminal fluid. The UPGMA dendrogram was generated using BioNumerics 5.1. (C) Control animals receiving only tropical forage; (R) supplemented animals receiving daily ruminal infusion of 230 g casein; (6 h) rumen fluid collected 6 h after the morning feeding; (18 h) rumen fluid collected 6 h after the evening feeding.Back to article page