Figure 4

Apoptosis-inducing factor (AIF) reduces burden of MTB in THP-1 cells but does not mediate the anti- MTB effects of IL-32γ. A) Western blot analysis for AIF in THP-1 cells transfected with scrambled siRNA and with siRNA that is complementary in sequence to AIF mRNA. Immunoblot and densitometry data shown are representative and the mean ± SD of three independent experiments, respectively. B) Apoptosis was quantified by TUNEL in THP-1 cells transfected with scrambled siRNA or AIF siRNA ± IL-32γ (50 ng/mL) ± MTB infection for 48 hours. Data shown are the mean ± SEM of three independent experiments. C) Immunoblot for AIF in THP-1 cells incubated with IL-32γ for 24 hours. Data shown are representative of three independent experiments. D) THP-1 cells transfected with scrambled siRNA or AIF siRNA were incubated with MTB ± IL-32γ (50 ng/mL) for four days and MTB quantified. Data shown are the mean ± SEM of three independent experiments. E) Immunoblot for AIF in THP-1 cells infected with MTB at the indicated times. Data shown are representative of three independent experiments. *p < 0.05, **p < 0.01.