Solution behavior of DsLOV measured by small angle X-ray scattering. (A) Experimental data (open circles), calculated curves of the crystal structure of the monomer (blue line), the crystal structure of the dimer (red line), and the crystal structure of the dimer including flexible ends (green line). The inset shows the zoom in on the low q-range, where a small fraction of aggregated particles cause the slight uptick at q < 0.024 Å−1. The lower panel shows the same experimental data and the fits in a Kratky-plot. (B) The distance distribution function P(r) of DsLOV as calculated for the range of q > 0.024 Å−1, where effects of protein aggregation are negligible. (C)
Ab initio model of DsLOV (cyan, shown in mesh) determined by SAXS and aligned crystal structure of the dimer (dimer orientation as in Figure 3A, two protein molecules are shown in coral and cyan with bound RBF molecules as stick model).