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Figure 6 | BMC Microbiology

Figure 6

From: Antibiosis interaction of Staphylococccus aureus on Aspergillus fumigatus assessed in vitro by mixed biofilm formation

Figure 6

Structural composition of ECM in single Aspergillus fumigatus and mixed A. fumigatus-Staphylococcus aureus in vitro biofilms by CLSM. A) co-localization of mannose and glucose/DNA, fluorochromes used are indicated on upper part. Overlapping images show white or intense yellow halos, evidencing molecular co-localization. A. fumigatus biofilm (100X) and mixed A. fumigatus-S. aureus biofilm (63X). B) co-localization of chitin/DNA, the fluorochrome used is indicated on upper part. Overlapping images show intense yellow halos, evidencing co-existence of chitin and DNA. White arrowheads indicate co-localization of macromolecules; yellow arrowheads indicate ECM; white arrow corresponds to hyphae with nule or scarce metabolic activity without concanavalin A (conA) signal.

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