Figure 1

Quantification and antagonism behavior of the single and mixed fungus-bacterium biofilm. A) Aspergillus fumigatus, Staphylococcus aureus, and A. fumigatus-S. aureus biofilm quantification. Biofilm quantification at different incubation times (0, 4, 8, 16, and 24 h). Biofilm biomass was quantified indirectly by the crystal violet method (see materials and methods). In plotting these data, the mean of the absorbance and standard error of the mean are relative to n = 10 measures, comparisons between absorbance (relatives to biomass biofilms formation) were made and significant differences were determined by Student-Newman-Keuls test, performing multicomparison of procedures and the following are indicated: (*), P < 0.050; and; (**), P < 0.001. Values are representative of three experiments with ten replicates each one. At right, see A. fumigatus, S. aureus and A. fumigatus-S. aureus micrographic of biofilm structure at 24 h. B) Evaluation of the antagonism behavior of S. aureus on Aspergillus fumigatus during mixed biofilm formation. Fungus or bacteria inoculums were placed, as first colonizer, allowing adherence 4 h, and the missing microorganism was added. Used values are representative of three experiments with ten replicates each one; when plotting data, mean absorbance and Standard error of the mean are relative to n = 10. Af = A. fumigatus; Sa = S. aureus; Af + Sa = A. fumigatus-S. aureus; Af4H + Sa = A. fumigatus with 4 previous h of adhesion plus S. aureus; Sa4H + A = S. aureus with 4 previous hours of adhesion plus A. fumigatus. Significant differences were determined by Student-Newman-Keuls test, performing a multicomparison of procedures; significant differences are indicated as: (*), P < 0.050.