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Table 2 Mitotype determination by PCR analysis (combination II)

From: Uniparental mitochondrial DNA inheritance is not affected in Ustilago maydis Δatg11 mutants blocked in mitophagy

Tumour sample 1

Spores analysed

LRI1 (F type) 2 (m1)

LRII1 (W type) 2 (m2)

  

a

b

c

d

a

b

c

d

Δatg11#1

8

0

0

0

6

8

0

0

0

Δatg11#2

10

0

0

0

5

10

0

0

0

Δatg11#3

21

0

0

0

8

21

0

0

0

Δatg11#4

10

0

0

0

6

10

0

0

0

Δatg11#5

9

0

0

0

1

9

0

0

0

Δatg11#6

11

0

0

0

3

11

0

0

0

Δatg11#7

11

0

0

0

3

11

0

0

0

Δatg11#8

11

0

0

0

2

11

0

0

0

Δatg11#9

11

0

0

0

1

11

0

0

0

Δatg11#10

10

0

0

0

1

10

0

0

0

Δatg11#11

11

0

0

0

3

11

0

0

0

Σ

123

0

0

0

39 (31.7) 3

123

0

0

0

wt #12

10

0

0

0

5

10

0

0

0

wt #13

10

0

0

0

2

10

0

0

0

wt #14

10

0

0

0

1

10

0

0

0

wt #15

9

0

0

0

2

9

0

0

0

wt #16

8

0

0

0

0

8

0

0

0

wt #17

11

0

0

0

3

11

0

0

0

wt #18

11

0

0

0

5

11

0

0

0

wt #19

11

0

0

0

3

11

0

0

0

wt #20

11

0

0

0

3

11

0

0

0

wt #21

11

0

0

0

5

11

0

0

0

wt #22

9

0

0

0

1

9

0

0

0

wt #23

11

0

0

0

2

11

0

0

0

Σ

122

0

0

0

32 (26.2) 3

122

0

0

0

  1. 1Wt strain combination: FB1/pMB2-2 x GF5/pKS1; Δatg11 strain combination: FB1Δatg11/pMB2-2 × GF5Δatg11/pKS1.
  2. 2Relative percentages of band intensities are indicated as: a) >97%, b) >50-97%, c) 3-50%, d) <3%.
  3. 3Values expressed in percentages (%).