Uniparental mitochondrial DNA inheritance is not affected in Ustilago maydis Δatg11 mutants blocked in mitophagy

Table 1 Mitotype determination by RFLP analysis (combination I)

Tumor sample ¹	Spores analysed	F type ³ (m2)				W type ³ (m1)				X1 type ³				F type dominance ⁴
		a	b	c	d	a	b	c	d	a	b	c	d
wt#1,#2²	8	8	0	0	0	0	0	0	1	0	0	0	0	100
wt#4	10	10	0	0	0	0	0	0	0	0	0	0	0	100
wt#5	9	9	0	0	0	0	0	0	4	0	0	0	1	100
wt#6	10	10	0	0	0	0	0	0	0	0	0	0	0	100
wt#7	10	3	0	0	0	0	0	0	6	7	0	0	0	30
wt#8	10	0	2	0	0	0	0	0	2	8	0	2	0	0
wt#9,#10²	9	9	0	0	0	0	0	0	0	0	0	0	0	100
Σ	66	49	2	0	0	0	0	0	13	15	0	2	1	74.2
Δatg11#1	9	8	0	0	0	0	0	0	1	1	0	0	0	88.9
Δatg11#2	10	10	0	0	0	0	0	0	4	0	0	0	0	100
Δatg11#4	8	7	0	1	0	0	0	0	0	0	1	0	1	87.5
Δatg11#7	10	7	0	0	0	3	0	0	0	0	0	0	0	70
Δatg11#8	10	5	0	0	0	4	0	0	0	1	0	0	0	50
Δatg11#11	10	10	0	0	0	0	0	0	1	0	0	0	0	100
Δatg11#12	10	0	0	0	0	0	0	0	0	10	0	0	0	0
Σ	67	47	0	1	0	7	0	0	6	12	1	0	1	70.1

¹Wt strain combination: MF34/pKS2 x FB2/pMB2-2; Δatg11 strain combination: MF34Δatg11/pKS2 x FB2Δatg11/pMB2-2.
²The values from these two samples were grouped.
³Relative percentages of band intensities are indicated as: a) >97%, b) >50-97%, c) 3-50%, d) <3%.
⁴Values (F type category a) expressed in percentages (%).

ISSN: 1471-2180