Figure 3From: Uniparental mitochondrial DNA inheritance is not affected in Ustilago maydis Δatg11 mutants blocked in mitophagy PCR analysis to detect mitotypes in wt and Δatg11 spores (combination II). A. PCR with DNA from individual spores from each three tumour samples of the wt (FB1/pMB2-2 x GF5/pKS1) and the Δatg11 mutant strain combination (FB1Δatg11/pMB2-2 x GF5Δatg11/pKS1). Labels ‘a’ and ‘b’ above the lanes refer to the primer combinations to detect the W and F type intron, respectively (see Figure 1). DNA from the parental strains K1 (FB1/pMB2-2), K3 (GF5/pKS1), K2 (FB1Δatg11/pMB2-2), and K4 (GF5Δatg11/pKS1) were included as controls. Open triangles mark faint bands (hardly detectable for probe 1/1) of the non-dominating F type (or W type in case of K1, K2; see Methods). DNA samples that were included in the RFLP analysis are marked by open circles (see Additional file 4: Figure S3). DNA samples that were additionally used for qPCR are marked by filled circles (see Additional file 5: Figure S4). Representative images are shown. B. Examples of peak profiles generated by ImageJ of faint F type signals shown in (A) and their absence in lanes lacking a corresponding signal. C. Boxplot of the data (expressed in percentages) shown in Table 2 (F type column d). Shown are the min./max. range (dots), median (horizontal line in the box), and the mean value (cross). The box contains ca. 50% of all data values ranging in both directions from the median. Note the increased variance (width of the box) for the Δatg11 mutant.Back to article page