Figure 6

Induced transcription of the member genes of RpoE2-regulated operons in the rpoE 2 null in-frame deletion mutant (MR-1ΔrpoE2) carrying the plasmid-borne rpoE2 gene. The strain carrying pHERD30T empty vector was used as control and 0.01% (w/v) of L-arabinose was added to the bacterial cultures of both control (carrying pHERD30T vector) and treatment (carrying pHERD30T-rpoE2) during late exponential phase (OD₆₀₀ > 0.8). The cells were collected for RNA extract after 1 hour of induction. A) Transcription of the genes was examined by using semi-quantitative RT-PCR; 16S rRNA gene exp ression was analyzed and used as the loading control. B) Trace quantity plotting of figure 6A using ‘Quantity One’ software.The quantitative data represents three times of assays in duplicates.