Table 2 Primers used in this study
From: CrdR function in a curdlan-producing Agrobacterium sp. ATCC31749 strain
Primer name | Oligonucleotide | Product length | Product name |
|---|---|---|---|
crdPG-1 | GTACTCGAGATTGTCGGCAGTCCAG | 607 | crdP |
crdPG-2 | AGCTCCTCGCCCTTGCTCACCATGAAATCAACTCCTCTGT | Â | Â |
GFP-1 | ACAGAGGAGTTGATTTCATGGTGAGCAAGGGCGAGGAGCT | 746 | GFP |
GFP-2 | CGCGGATCCTTACTTGTACAGCTCGTCCATG | Â | crdP |
crdP-1 | TCACCAACACCAACTCTGGA | Â | Â |
crdP-2 | CATGAAATCAACTCCTCTGT | 607 | crdP |
crdP142-1 | ATCGTCAGATGCCTATTTGT | 537 | crdP |
crdP108-1 | AAATTAGTTAATGCAAT | 503 | crdP |
crdP98-1 | TTAATGCAATTTTTACTATGTT | 493 | crdP |
crdP53-1 | CCATTTCAATACTGCGGGAGG | 448 | crdP |
crdP13-1 | AGGAGTTGATTTCATGCTGTT | 408 | crdP |
crdP1-1 | ATGCTGTTCCGCAATAAG | 395 | crdA |
crdA395-2 | TCGGTCCGCAGCAGCAAAG | Â | Â |
q-crdA-1 | CAAGGCATAAGCGAAGACATC | 227 | crdA |
q-crdA-2 | CTCCGTGTTTCAAGTGTGGTC | Â | Â |
q-crdS-1 | AACCTGACGATTGCGATTGGG | 179 | crdS |
q-crdS-2 | GTGTAGCACCAGAGCGTTTCG | Â | Â |
q-crdC-1 | GTTCGGTCAGGATGCTCAAC | 248 | crdC |
q-crdC-2 | GCCAAAGTTCGGAATCAATG | Â | Â |
crdR-1 | GCCAGATCTATGACCGAGAATAAGAAAAAGCCT | 437 | crdR |
crdR-2 | TTGAGCTCTTACTCGGCGTCGCCTTCG | Â | Â |
NcrdR-1 | ACACTCGAGATACACCCGGTCCCTACCAGCATT | Â | crdR |
NcrdR-2 | TTTGAGCTCCTTGTCCTTCTTCAGAAGCGTGT | 1302 | crdR |
q-crdR-1 | TCGGAATGAGCCAGGAGAAGC | 238 | crdR |
q-crdR-2 | TCAGCCGAGGACAGAAAGTCG | Â | Â |
crdRup-1 | TCTGAGCTCTTCGGCGTTTCGGAATGGTTG | 2533 | CrdR |
crdRdown-2 | CAACACAAGCTTAACCGTCACCTGGCTCTTGGCA | Â | Â |
crdRcheckGm-2 | TTGGGCATACGGGAAGAAGT | 558 | Gm |
crdRcheckGm-1 | CGGCTGATGTTGGGAGTAGG | 577 | Â |
Rep-Kan-1 | ACGCGTCGACCTTGCCAGCCCGTGGATAT | 3266 | Km |
Rep-Kan-2 | ACGCGTCGACTCTGTGATGGCTTCCATGTC | Â | Â |
Gm-1 | ATAGTTGTCGAGATATTCACTAGTCGTCAGGTGGCACTTTTCG | 1302 | Gm |
Gm-2 | CGCGGATCCGCTCTGCTGAAGCCAGTTAC | Â | Â |
celAP1 | ACCCGGTCTATCCCATGA | Â | Â |
celAP-2 | CATCCAGAAACTTTCCGT | 441 | celAP |
relAP-1 | CCAGATTTCTCAAGGGTC | Â | Â |
RelAP-2 | CATCATGCGATATTCCACA | 443 | relAP |
crdRP-1 | GCGGCGATCCTAAATGTGAC | Â | Â |
crdRP-2 | CATGCGGTCCTGACACTCG | 466 | crdRP |
crdSP-1 | TCGGTCGGCACATGGGTCAAT | Â | Â |
crdSP-2 | CATCGCCCTAACCTCGCAGT | 446 | crdSP |