Table 1 Bacterial strains and plasmids used in this study
From: CrdR function in a curdlan-producing Agrobacterium sp. ATCC31749 strain
Strain/plasmids | Description | Sources |
|---|---|---|
Strains | ||
E.coli BL21 | Res − Mod − ompT (DE3 with T7 pol) (pLysS with T7 lysozyme;Cm r) Novagen | Lab stock |
E.coli TG1 | Cloning host | TaKaRa |
ATCC31749 | Curdlan-producing Agrobacterium sp. (wild-type strain) | ATCC |
ATCC31749ΔcrdR | ATCC 31749 mutant with gene knockout of crdR | This study |
Plasmids | ||
pEGFP | GFP expression vector | Clontech |
pQE81L | Expression vector, Amp R | Qiagen |
pBBR122 | Gram-negative broad host vector | MoBiTec |
pEX18Gm | Expression vector carrying sacBR, Gm R | [36] |
pBQ | Vector derived from both pQE80 and pBBR122 | [37] |
pUC19 | Cloning vector | TaKaRa |
pUC19T-crdR | Suicide vector for crdR knock-out | This study |
pBQcrdR | Expression vector with T 5 driving crdR expression | This study |
pBQNcrdR | Expression vector with crdRP driving crdR expression | This study |
pAG | Expression vector with GFP driven by crd promoter | This study |
pMD18-T(crdA) | Derivative of pMD18-T with part of crdA | This study |
pMD18-T(crdS) | Derivative of pMD18-T with part of crdS | This study |
pMD18-T(crdC) | Derivative of pMD18-T with part of crdC | This study |
pMD18-T(crdR) | Derivative of pMD18-T with part of crdR | This study |