Cleavage of the moaX-encoded fused molybdopterin synthase from Mycobacterium tuberculosis is necessary for activity

Table 1 The ability of MPT-synthase encoding genes from M. tuberculosis to restore MoCo biosynthesis in the M. smegmatis ΔmoaD2 ΔmoaE2 mutant

MPT-synthase-encoding gene/s	Vector	Functional complementation*
Mtb moaD1 + Mtb moaE1	Integration	No
Mtb moaD1 + Mtb moaE2	Integration	No
Mtb moaD2 + Mtb moaE1	Integration	No
Mtb moaD2 + Mtb moaE2	Integration	No
Mtb moaD1+ Mtb moaE1	Episomal	No
Mtb moaD1+ Mtb moaE2	Episomal	No
Mtb moaD2+ Mtb moaE1	Episomal	No
Mtb moaD2 + Mtb moaE2	Episomal	Yes
moaX	Integration	Yes
moaX	Episomal	Yes
moaXFL	Episomal^§	Yes
moaXFL ^G81A	Episomal^§	No
moaXFL ^G82A	Episomal^§	No

*As measured by the ability to restore growth of the M. smegmatis ΔmoaD2 ΔmoaE2 mutant on nitrate as sole nitrogen source. ^§Carried on pFLAGEM, with a C-terminal 3 × FLAG-tag.

ISSN: 1471-2180