Figure 5From: Cleavage of the moaX-encoded fused molybdopterin synthase from Mycobacterium tuberculosis is necessary for activity Western blot analysis of MoaXFL in E. coli . Included as a positive control is protein extract from M. smegmatis expressing moaX from the same system. The correct 26.5Â kDa band corresponding to un-cleaved MoaX is observed in both organisms. However, the 17.8Â kDa cleavage product corresponding to the MoaE component of MoaX is not observed in E. coli, which displays a larger (~20Â kDa) band.Back to article page