Skip to main content
Figure 4 | BMC Microbiology

Figure 4

From: Cleavage of the moaX-encoded fused molybdopterin synthase from Mycobacterium tuberculosis is necessary for activity

Figure 4

Cleavage of MoaX is necessary for function. (A) Nitrate assimilation in strains carrying FLAG-tagged mutated derivatives of MoaX. Shown are strains carrying either a G81A or G82A mutation. (B) Western blot analysis. An antibody directed at the FLAG-tag was used (C) Nitrate assimilation in the ΔmoaE2 single mutant of M. smegmatis. Growth curves were performed in MPLN medium with cell density measured over a period of 5 days. The G81A mutation abolished cleavage, whereas mutation G82A did not interfere with processing, but yielded a non-functional MoaD subunit. All data represent the result of three independent experiments.

Back to article page