Figure 2

Analysis of gene expression and MoCo biosynthesis in genetically complemented strains. (A) Comparison of heterologous gene expression levels from integrating (single copy) versus episomal (multicopy) complementing vectors. The expression level of each gene was normalized against the expression of the sigA gene. In all cases, the data shown are representative of three independent biological replicates with standard errors of the mean. The expression of moaD2 and moaE2 in wild type (WT) M. smegmatis and the expression of moaX, when provided in single copy (pTX) or multicopy (pMX) was also assessed for comparison. (B) Nitrate assimilation in strains complemented with episomal vectors carrying different combinations of MPT-synthase-encoding genes. Strains were grown in MPLN medium supplemented with 10 mM Nitrate as the sole nitrate source.