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Table 2 Bacterial strains

From: Tn5/7-lux: a versatile tool for the identification and capture of promoters in Gram-negative bacteria

Strain

Genotype or relevant features

Source

E. coli

DH5α

F−φ80 lacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK −mK +) phoA glnV44

[49]

MaH1

F−φ80 lacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK −mK +) phoA glnV44 attTn7::pir116+

[50]

RHO3

thi-1 thr-1 leuB26 tonA21 lacY1 supE44 recA integrated RP4-2 Tcr::Mu (λpir +)Δasd::FRT ΔaphA::FRT

[51]

RHO5

attTn7::pir116 + thi-1 thr-1 leuB26 tonA21 lacY1 supE44 recA integrated RP4-2 Tcr::Mu (λpir +)Δasd::FRT ΔaphA::FRT

[50]

KVT9

DH5α rbsC::mini-Tn5/7LuxK3

This Study

KVT11

DH5α attTn7::mini-Tn7-P DH51 -lux

This Study

KVT12

DH5α attTn7::mini-Tn7-P rbs -lux

This Study

B. pseudomallei

Bp82

1026b ΔpurM

[52]

Bp82.27

Bp82 Δ(amrRAB-oprA)

Laboratory collection

Bp82.68

Bp82.27 attTn7::mini-Tn7-P ompA -lux (glmS1)

This Study

Bp82.69

Bp82.27 attTn7::mini-Tn7-P tolC -lux (glmS1)

This Study

Bp82.70

Bp82.27attTn7::mini-Tn7-P PA4974 -lux (glmS2 + glmS3)

This Study

Bp82.77

Bp82.27attTn7::mini-Tn7-P ompA -lux (glmS1 + glmS2)

This Study

Bp82.78

Bp82.27 attTn7::mini-Tn7-P ompA -lux (glmS1)

This Study

Bp82.79

Bp82.27 attTn7::mini-Tn7-P ompA -lux (glmS2 + glmS3)

This Study

Bp82.80

Bp82.27 attTn7::mini-Tn7-P ompA -lux (glmS2)

This Study

Bp82.81

Bp82.27 attTn7::mini-Tn7-P ompA -lux (glmS3)

This Study

A. baumannii

ATCC19606

Prototroph

American Type Culture Collection

IFD1

ATCC19606 A1S_2680::mini-Tn5/7LuxK5

This Study

IFD2

ATCC19606 A1S_2773::mini-Tn5/7LuxK5

This Study

IFD3

ATCC19606 A1S_0947::mini-Tn5/7LuxK5 and A1S_2736::Tn5/7LuxK5

This Study

IFD4

ATCC19606 A1S_2621::mini-Tn5/7LuxK5

This Study

IFD5

ATCC19606 attTn7::mini-Tn7-P H1A -LuxK5

This Study