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Figure 3 | BMC Microbiology

Figure 3

From: Tn5/7-lux: a versatile tool for the identification and capture of promoters in Gram-negative bacteria

Figure 3

Map of mini-Tn 5/7 - lux delivery plasmid pTn 5/7 LuxK3. The mini-Tn5/7-lux element flanked by the Tn5 mosaic ends (ME) carries a promoter-less P. luminescens luxCDABE operon, the kanamycin resistance encoding nptII gene, the Tn7 left (Tn7L) and right (Tn7R) ends, the R6K origin of replication (ori R6K), and an origin of conjugative transfer (oriT). The Tn5 transposase-encoding tnpA gene is located outside of the transposable element. Other abbreviations: FRT, single Flp recombinase target site; T0T1, transcriptional terminators T0 and T1 from bacteriophage λ and E. coli rrnB operon, respectively.

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BMC Microbiology

ISSN: 1471-2180

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