Figure 2From: The truth about metagenomics: quantifying and counteracting bias in 16S rRNA studies Observed bias by bacterium. The observed bias (the observed minus the actual proportions) for each bacterium in the experimental design due to the different effects of our DNA Extraction, PCR amplification, and sequencing and taxonomic classification protocols. The total bias is also plotted for each bacterium. For each box and whisker plot, only the samples including the bacterium were included.Back to article page