Expression of extra-cellular levansucrase in Pseudomonas syringae is controlled by the in planta fitness-promoting metabolic repressor HexR

Table 2 Oligonucleotide primers used in this study

Oligonucleotides	Nucleotide sequence (5′ to 3′)
HR_1f^a	GGATCC GTTCAACTCATCGAGTC
HR_1r	CAGATGCGACTGTTCGTC
HR_2f	GACCCCCGGATCAGTGCCAG
HR_2r^a	GGATCC GGTACC CAGCCGCTATCCGATCGAG
lscB/C_RT_fwd	TCGGTTATCCTGACCCTGAC
lscB/C_RT_rev	CCATGACGATCTTCCCAGTC
lscB_hex_fwd	CGCAATTAATGCGAGCCCGCAGG
lscB_hex_rev	TTGCATTGGTCTCCTTGTGCTTC
hex_up_fwd	CGAGCAAGTCGCACCG
hex_down_rev	GAAGTCGACATGCAGGTAG
hexR_fwd	GAATTCATGGACCGCGTAAGAAACC
hexR_rev	CTGCAGTCAGCCTTGATCCTCGATC
hexR_verif_fwd	CTCAACCCGCAGATGGCAA
hexR_verif_rev	CGATGACCTCGCGGATCAT

^aRestriction sites in the primers are in italics: GGATCC - BamHI, GGTACC - KpnI.

ISSN: 1471-2180