Strain | Relevant characteristics a | Reference/source |
---|---|---|
Escherichia coli | ||
DH5α | supE44 ΔlacU169 (Φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | [26] |
Pseudomonas syringae pv. Glycinea | ||
PG4180 | wild type, levan+ | [29] |
hexR | Kmr, hexR mutant of PG4180, levan++ | This study |
Plasmid | Â | Â |
pRK2013 | Kmr, helper plasmid | [30] |
pBBR1MCS | Cmr, broad-host-range cloning vector | [31] |
pBBR1MCS-3 | Tcr, broad-host-range cloning vector | [32] |
pGEM-T Easy | Apr, vector for cloning of PCR products | Promega |
pEX18Ap | Apr, oriT+ sacB+ gene replacement vector | [33] |
pFKm | Source of Km cassette flanked with FRT sequences | [34] |
pGEM.hexR1 | Apr, contains 456-bp upstream region of hexR | This study |
pGEM.hexR2 | Apr, contains 360-bp downstream region of hexR | This study |
pGEM.hexR1-Km | Apr, Km, 1230-bp KpnI fragment containing Km-FRT cassette of pFKm1 cloned into pGEM.hexR1 | This study |
pGEM.hexR-Km | Apr, Km, 360-bp BamHI-SpeI fragment of pGEM.hexR2 cloned into pGEM.hexR1-Km | This study |
pEX.hexR-Km | Apr, Km, 2046-bp EcoRI fragment of pGEM.hexR-Km cloned into pEX18Ap | This study |