Dissemination of IncF-type plasmids in multiresistant CTX-M-15-producing Enterobacteriaceae isolates from surgical-site infections in Bangui, Central African Republic

Rafaï, Clotaire; Frank, Thierry; Manirakiza, Alexandre; Gaudeuille, Alfred; Mbecko, Jean-Robert; Nghario, Luc; Serdouma, Eugene; Tekpa, Bertrand; Garin, Benoit; Breurec, Sebastien

doi:10.1186/s12866-015-0348-1

Table 4 Resistance genes transferred to transconjugants and electroporants and their replicon type

From: Dissemination of IncF-type plasmids in multiresistant CTX-M-15-producing Enterobacteriaceae isolates from surgical-site infections in Bangui, Central African Republic

Species	ST	ERIC and REP-PCR based pattern ^a	Hospital/department	PMQR determinants ^b	Transconjugant or transformant ^c
Species	ST	ERIC and REP-PCR based pattern ^a	Hospital/department	PMQR determinants ^b	Incompatibility group	FII:FIA:FI formula	PMQR determinants ^b	Non-beta-lactam drug resistance
Escherichia coli	10	A	A/GS	QnrB, QnrS, AAC(6′)-1b-cr	IncF	F31:A4:B1	QnrB, AAC(6′)-1b-cr	GEN, TOB, NAL
E. coli	10	A	A/U	QnrB, AAC(6′)-1b-cr	IncF	F31:A4:B1	QnrB, AAC(6′)-1b-cr	GEN, TOB, NAL
E. coli	10	B	C/O	QnrB, QnrS, AAC(6′)-1b-cr	IncF	F31:A4:B1	AAC(6′)-1b-cr	KAN, GEN, TOB, CHL, TET, SUL, SXT
E. coli	10	C	P/GS	QnrS, AAC(6′)-1b-cr	IncF	F31:A4:B1	AAC(6′)-1b-cr	GEN, TOB, CHL, TET
E. coli	10	D	C/O	QnrB, QnrS, AAC(6′)-1b-cr	IncF	F31:A4:B1	AAC(6′)-1b-cr	KAN, GEN, TOB, CHL, TET, SUL, SXT
E. coli	146	E	A/GS	QnrB, QnrS, AAC(6′)-1b-cr	IncF	F36:A4:B1	AAC(6′)-1b-cr	GEN, TOB, CHL, TET
E. coli	131	F	A/GS	QnrB, AAC(6′)-1b-cr	IncF	F31:A4:B1	QnrB, AAC(6′)-1b-cr	KAN, GEN, TOB, CHL, NAL, TET, SUL, SXT
E. coli	131	G	A/GS	QnrB, QnrS, AAC(6′)-1b-cr	IncF	F31:A4:B1	QnrB, AAC(6′)-1b-cr	KAN, GEN, TOB, CHL, NAL, TET, SUL, SXT
E. coli	131	H	A/GS	QnrB, QnrS, AAC(6′)-1b-cr	IncF	F31:A4:B1	AAC(6′)-1b-cr	GEN, TOB, STR, TET, SUL, SXT
E. coli	131	I	A/GS	QnrB, QnrS, AAC(6′)-1b-cr	IncF	F1:A1:B20	AAC(6′)-1b-cr	GEN, TOB, CHL, TET, SXT
E. coli	131	J	A/GS	QnrB, QnrS, AAC(6′)-1b-cr	IncF	F36:A4:B1	AAC(6′)-1b-cr	KAN, GEN, TOB, NET, STR, CHL, TET, SUL, SXT
E. coli	131	J	C/O	AAC(6′)-1b-cr	IncF	F31:A4:B1	AAC(6′)-1b-cr	KAN, GEN, TOB, NET, STR, CHL, TET, SUL, SXT
E. coli	131	K	A/U	QnrB, QnrS, AAC(6′)-1b-cr	IncF	F31:A4:B1	QnrB, AAC(6′)-1b-cr	KAN, GEN, TOB, CHL, NAL, TET, SUL, SXT
E. coli	156	L	CP/GS	QnrB, QnrS, AAC(6′)-1b-cr	IncF	Fnew:A-:B20	AAC(6′)-1b-cr	KAN, GEN, TOB,SUL, SXT
E. coli	354	M	CP/GS	QnrS, AAC(6′)-1b-cr	IncF	F1:A1:B1	AAC(6′)-1b-cr	KAN, GEN, TOB, NET, STR, TET, SUL, SXT
E. coli	405	N	CP/GS	QnrB, QnrS, AAC(6′)-1b-cr	IncF	F31:A4:B1	AAC(6′)-1b-cr	GEN, TOB, CHL, TET
E. coli	405	O	A/GS	QnrB, QnrS, AAC(6′)-1b-cr	IncF	F36:A4:B1	QnrB, AAC(6′)-1b-cr	KAN, GEN, TOB, CHL, NAL, TET, SUL, SXT
Enterobacter cloacae	^d	A	A/GS	QnrB, QnrS, AAC(6′)-1bcr	IncHI2	Not tested	QnrB, AAC(6′)-1bcr	KAN, STR, NAL, TET, SUL, SXT
Enterobacter cloacae	^d	B	CP/GS	QnrB, QnrS, AAC(6′)-1bcr	IncF	Fnew:A-:B20	AAC(6′)-1bcr	KAN, TOB, STR, TET, SUL, SXT
Enterobacter cloacae	^d	C	A/G	QnrB, QnrS, AAC(6′)-1bcr	IncF	F31:A4:B1	AAC(6′)-1bcr	GEN, TOB, STR, CHL, TET, SUL, SXT
Enterobacter cloacae	^d	D	A/G	QnrB, QnrS, AAC(6′)-1bcr	IncF	F31:A4:B1	QnrB, AAC(6′)-1bcr	GEN, TOB, STR, CHL, NAL, TET, SUL, SXT
Enterobacter cloacae	^d	E	C/O	QnrB, QnrS, AAC(6′)-1bcr	TF^e
Enterobacter cloacae	^d	F	C/O	QnrB, QnrS, AAC(6′)-1bcr	IncF	F36:A4:B1	QnrB, AAC(6′)-1bcr	GEN, TOB, STR, CHL, NAL, TET, SUL, SXT
Klebsiella pneumoniae	^d	A	A/U	QnrB, AAC(6′)-1bcr	TF
K. pneumoniae	^d	B	C/O	QnrB, AAC(6′)-1bcr	IncF	K4:- :B-	QnrB, AAC(6′)-1bcr	GEN, TOB, STR, NAL, TET, SUL, SXT
K. pneumoniae	^d	C	CP/GS	QnrB, QnrS, AAC(6′)-1bcr	IncF	K4:- :B-	QnrB, AAC(6′)-1bcr	GEN, TOB, STR, NAL, TET, SUL, SXT
K. pneumoniae	^d	D	A/G	QnrS, AAC(6′)-1bcr	TF
K. pneumoniae	^d	E	A/G	QnrB, AAC(6′)-1bcr	TF
Proteus mirabilis	^d	A	A/GS	QnrB, QnrS, AAC(6′)-1bcr	NT^f
P. mirabilis	^d	B	C/O	QnrS, AAC(6′)-1bcr	NT
Enterobacter amnigenus	^d	A	A/G	QnrB, QnrS, AAC(6′)-1bcr	IncF	F31:A4:B1	AAC(6′)-1bcr	GEN, TOB, TET
Enterobacter sakazakii	^d	A	C/O	QnrB, QnrS, AAC(6′)-1bcr	TF
K. oxytoca	^d	A	A/G	QnrB, QnrS, AAC(6′)-1bcr	TF
Morganella morganii	^d	A	C/O	QnrS, AAC(6′)-1bcr	TF

TF, transfer to the E. coli recipient failed; NT, the plasmid could not be typed; ST, sequence type; PMQR, plasmid-mediated quinolone resistance, A/U, Amitié Hospital/urology; C/O, Community Hospital/orthopaedics; CP/GS, Complexe Pédiatrique/general surgery; A/GS, Amitié Hospital/general surgery; A/G, Amitié Hospital/gynaecology; KAN, kanamycin; GEN, gentamicin; TOB, tobramycin; NET, netilmicin; SRT, streptomycin; CHL, chloramphenicol; NA, nalidixic acid; TET, tetracycline; SUL, sulfamide; SXT, co-trimoxazole.
^aResistance transfer experiments were carried out on one randomly selected strain representative from all resistance gene content within each Rep- and ERIC-PCR banding pattern.
^bQepA were not found in any of the isolates.
^c bla _CTX-M-15 was found in all transconjugants and transformants.
^dnot tested.
^eTF indicates that the transfer in the E. coli recipient failed.
^fNT indicates that the plasmid was not typable.

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ISSN: 1471-2180

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