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Table 4 Resistance genes transferred to transconjugants and electroporants and their replicon type

From: Dissemination of IncF-type plasmids in multiresistant CTX-M-15-producing Enterobacteriaceae isolates from surgical-site infections in Bangui, Central African Republic

Species ST ERIC and REP-PCR based pattern a Hospital/department PMQR determinants b Transconjugant or transformant c
Incompatibility group FII:FIA:FI formula PMQR determinants b Non-beta-lactam drug resistance
Escherichia coli 10 A A/GS QnrB, QnrS, AAC(6′)-1b-cr IncF F31:A4:B1 QnrB, AAC(6′)-1b-cr GEN, TOB, NAL
E. coli 10 A A/U QnrB, AAC(6′)-1b-cr IncF F31:A4:B1 QnrB, AAC(6′)-1b-cr GEN, TOB, NAL
E. coli 10 B C/O QnrB, QnrS, AAC(6′)-1b-cr IncF F31:A4:B1 AAC(6′)-1b-cr KAN, GEN, TOB, CHL, TET, SUL, SXT
E. coli 10 C P/GS QnrS, AAC(6′)-1b-cr IncF F31:A4:B1 AAC(6′)-1b-cr GEN, TOB, CHL, TET
E. coli 10 D C/O QnrB, QnrS, AAC(6′)-1b-cr IncF F31:A4:B1 AAC(6′)-1b-cr KAN, GEN, TOB, CHL, TET, SUL, SXT
E. coli 146 E A/GS QnrB, QnrS, AAC(6′)-1b-cr IncF F36:A4:B1 AAC(6′)-1b-cr GEN, TOB, CHL, TET
E. coli 131 F A/GS QnrB, AAC(6′)-1b-cr IncF F31:A4:B1 QnrB, AAC(6′)-1b-cr KAN, GEN, TOB, CHL, NAL, TET, SUL, SXT
E. coli 131 G A/GS QnrB, QnrS, AAC(6′)-1b-cr IncF F31:A4:B1 QnrB, AAC(6′)-1b-cr KAN, GEN, TOB, CHL, NAL, TET, SUL, SXT
E. coli 131 H A/GS QnrB, QnrS, AAC(6′)-1b-cr IncF F31:A4:B1 AAC(6′)-1b-cr GEN, TOB, STR, TET, SUL, SXT
E. coli 131 I A/GS QnrB, QnrS, AAC(6′)-1b-cr IncF F1:A1:B20 AAC(6′)-1b-cr GEN, TOB, CHL, TET, SXT
E. coli 131 J A/GS QnrB, QnrS, AAC(6′)-1b-cr IncF F36:A4:B1 AAC(6′)-1b-cr KAN, GEN, TOB, NET, STR, CHL, TET, SUL, SXT
E. coli 131 J C/O AAC(6′)-1b-cr IncF F31:A4:B1 AAC(6′)-1b-cr KAN, GEN, TOB, NET, STR, CHL, TET, SUL, SXT
E. coli 131 K A/U QnrB, QnrS, AAC(6′)-1b-cr IncF F31:A4:B1 QnrB, AAC(6′)-1b-cr KAN, GEN, TOB, CHL, NAL, TET, SUL, SXT
E. coli 156 L CP/GS QnrB, QnrS, AAC(6′)-1b-cr IncF Fnew:A-:B20 AAC(6′)-1b-cr KAN, GEN, TOB,SUL, SXT
E. coli 354 M CP/GS QnrS, AAC(6′)-1b-cr IncF F1:A1:B1 AAC(6′)-1b-cr KAN, GEN, TOB, NET, STR, TET, SUL, SXT
E. coli 405 N CP/GS QnrB, QnrS, AAC(6′)-1b-cr IncF F31:A4:B1 AAC(6′)-1b-cr GEN, TOB, CHL, TET
E. coli 405 O A/GS QnrB, QnrS, AAC(6′)-1b-cr IncF F36:A4:B1 QnrB, AAC(6′)-1b-cr KAN, GEN, TOB, CHL, NAL, TET, SUL, SXT
Enterobacter cloacae d A A/GS QnrB, QnrS, AAC(6′)-1bcr IncHI2 Not tested QnrB, AAC(6′)-1bcr KAN, STR, NAL, TET, SUL, SXT
Enterobacter cloacae d B CP/GS QnrB, QnrS, AAC(6′)-1bcr IncF Fnew:A-:B20 AAC(6′)-1bcr KAN, TOB, STR, TET, SUL, SXT
Enterobacter cloacae d C A/G QnrB, QnrS, AAC(6′)-1bcr IncF F31:A4:B1 AAC(6′)-1bcr GEN, TOB, STR, CHL, TET, SUL, SXT
Enterobacter cloacae d D A/G QnrB, QnrS, AAC(6′)-1bcr IncF F31:A4:B1 QnrB, AAC(6′)-1bcr GEN, TOB, STR, CHL, NAL, TET, SUL, SXT
Enterobacter cloacae d E C/O QnrB, QnrS, AAC(6′)-1bcr TFe    
Enterobacter cloacae d F C/O QnrB, QnrS, AAC(6′)-1bcr IncF F36:A4:B1 QnrB, AAC(6′)-1bcr GEN, TOB, STR, CHL, NAL, TET, SUL, SXT
Klebsiella pneumoniae d A A/U QnrB, AAC(6′)-1bcr TF    
K. pneumoniae d B C/O QnrB, AAC(6′)-1bcr IncF K4:- :B- QnrB, AAC(6′)-1bcr GEN, TOB, STR, NAL, TET, SUL, SXT
K. pneumoniae d C CP/GS QnrB, QnrS, AAC(6′)-1bcr IncF K4:- :B- QnrB, AAC(6′)-1bcr GEN, TOB, STR, NAL, TET, SUL, SXT
K. pneumoniae d D A/G QnrS, AAC(6′)-1bcr TF    
K. pneumoniae d E A/G QnrB, AAC(6′)-1bcr TF    
Proteus mirabilis d A A/GS QnrB, QnrS, AAC(6′)-1bcr NTf    
P. mirabilis d B C/O QnrS, AAC(6′)-1bcr NT    
Enterobacter amnigenus d A A/G QnrB, QnrS, AAC(6′)-1bcr IncF F31:A4:B1 AAC(6′)-1bcr GEN, TOB, TET
Enterobacter sakazakii d A C/O QnrB, QnrS, AAC(6′)-1bcr TF    
K. oxytoca d A A/G QnrB, QnrS, AAC(6′)-1bcr TF    
Morganella morganii d A C/O QnrS, AAC(6′)-1bcr TF    
  1. TF, transfer to the E. coli recipient failed; NT, the plasmid could not be typed; ST, sequence type; PMQR, plasmid-mediated quinolone resistance, A/U, Amitié Hospital/urology; C/O, Community Hospital/orthopaedics; CP/GS, Complexe Pédiatrique/general surgery; A/GS, Amitié Hospital/general surgery; A/G, Amitié Hospital/gynaecology; KAN, kanamycin; GEN, gentamicin; TOB, tobramycin; NET, netilmicin; SRT, streptomycin; CHL, chloramphenicol; NA, nalidixic acid; TET, tetracycline; SUL, sulfamide; SXT, co-trimoxazole.
  2. aResistance transfer experiments were carried out on one randomly selected strain representative from all resistance gene content within each Rep- and ERIC-PCR banding pattern.
  3. bQepA were not found in any of the isolates.
  4. c bla CTX-M-15 was found in all transconjugants and transformants.
  5. dnot tested.
  6. eTF indicates that the transfer in the E. coli recipient failed.
  7. fNT indicates that the plasmid was not typable.