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Table 5 E. coli strains

From: A new suite of tnaA mutants suggests that Escherichia coli tryptophanase is regulated by intracellular sequestration and by occlusion of its active site

Strain

Description a

Source or Reference

MG1655

Wild type F λ ilvG rfb-50 rph-1

Laboratory collection

GL38

MG1655 aer-sfgfp::kan

[25]

GL40

MG1655 tnaA-sfgfp::kan

[25]

GL60

MG1655 ibpA-mCherry::kan

[25]

GL504

MG1655 ΔtnaA::frt

[25]

GL619

GL607 ΔtnaAB::frt PtufA::tnaA-sfgfp tnaB::frt

[19]

GL676

MG1655 tnaA-mgfpmut3::kan

This study

GL678

MG1655 D1-sfgfp::kan

This study

GL680

MG1655 D1D2-sfgfp::kan

This study

GL682

GL504 D3-sfgfp::kan

This study

GL684

GL504 D2D3-sfgfp::kan

This study

GL686

GL504 D1D3-sfgfp::kan

This study

GL688

GL504 D2-sfgfp::kan

This study

GL726

GL686 D1D3-sfgfp::frt

This study

GL730

GL726 D1D3-sfgfp::frt ibpA-mCherry::kan

This study

GL734

GL504 D1D3-mgfpmut3::kan

This study

GL801

GL504 A1-tnaA-sfgfp::kan (K5A)

This study

GL802

GL504 A2-tnaA-sfgfp::kan (E9A, R12A, R14A)

This study

GL803

GL504 A3-tnaA-sfgfp::kan (E17A, K20A, R21A)

This study

GL804

GL504 A4-tnaA-sfgfp::kan (T23A, R24A, Y26A)

This study

GL805

GL504 A5-tnaA-sfgfp::kan (R27A, E28A, E29A)

This study

GL806

GL504 A6-tnaA-sfgfp::kan (K33A, S34A)

This study

GL807

GL504 A7-tnaA-sfgfp::kan (D42A, S43A, E44A, D45A)

This study

GL808

GL504 A8-tnaA-sfgfp::kan (D49A, T52A, D53A, S54A)

This study

GL809

GL504 A9-tnaA-sfgfp::kan (T60A, Q61A, S62A, Q64A)

This study

GL810

GL504 A10-tnaA-sfgfp::kan (K115A)

This study

GL811

GL504 A11-tnaA-sfgfp::kan (K156A)

This study

GL812

GL504 A12-tnaA-sfgfp::kan (K239A)

This study

GL813

GL504 A13-tnaA-sfgfp::kan (K270A)

This study

GL814

GL504 A14-tnaA-sfgfp::kan (N327A, D329A)

This study

GL815

GL504 A15-tnaA-sfgfp::kan (Q339A, Y340A, D343A)

This study

GL816

GL504 A16-tnaA-sfgfp::kan (E346A, E347A)

This study

GL817

GL504 A17-tnaA-sfgfp::kan (C352A, Q353A, Q354A)

This study

GL818

GL504 A18-tnaA-sfgfp::kan (D363A, K366A)

This study

GL819

GL504 A19-tnaA-sfgfp::kan (H370A, D374A, Q375A)

This study

GL820

GL504 A20-tnaA-sfgfp::kan (E384A, K387A, R392A)

This study

GL821

GL504 A21-tnaA-sfgfp::kan (S398A, R403A, D404A)

This study

GL822

GL504 A22-tnaA-sfgfp::kan (K406A, K409A, Q410A)

This study

GL823

GL504 A23-tnaA-sfgfp::kan (E416A, R419A)

This study

GL824

GL504 A24-tnaA-sfgfp::kan (T426A, Y427A, T428A)

This study

GL825

GL504 A25-tnaA-sfgfp::kan (Q429A, T430A, H431A, D433A)

This study

GL826

GL504 A26-tnaA-sfgfp::kan (E437A, K440A, H441A)

This study

GL827

GL504 A27-tnaA-sfgfp::kan (K443A, E444A, N445A, N448A)

This study

GL828

GL504 A28-tnaA-sfgfp::kan (K450A)

This study

GL829

GL504 A29-tnaA-sfgfp::kan (T453A, T455A, Y456A, E457A)

This study

GL830

GL504 A30-tnaA-sfgfp::kan (K459A)

This study

GL831

GL504 A31-tnaA-sfgfp::kan (R462A, H463A, T465A)

This study

GL832

MG1655 A32-tnaA-sfgfp::kan (K467A, K469A, E470A)

This study

GL833

GL504 A21a-tnaA-sfgfp::kan (S398A)

This study

GL834

GL504 A21b-tnaA-sfgfp::kan (R403A)

This study

GL835

GL504 A21c-tnaA-sfgfp::kan (D404A)

This study

GL836

GL504 A23a-tnaA-sfgfp::kan (E416A)

This study

GL837

GL504 A23b-tnaA-sfgfp::kan (R419A)

This study

GL838

GL504 A31a-tnaA-sfgfp::kan (R462A)

This study

GL839

GL504 A31b-tnaA-sfgfp::kan (H463A)

This study

GL840

GL504 A31c-tnaA-sfgfp::kan (T465A)

This study

GL844

GL504 A33-tnaA-sfgfp::kan (K5A, K115A, K156A, K239A, K450A, K459A)

This study

GL802D

GL504 A2-D1D3-sfgfp::kan (E9A, R12A, R14A)

This study

GL803D

GL504 A3-D1D3-sfgfp::kan (E17A, K20A, R21A)

This study

GL804D

GL504 A4-D1D3-sfgfp::kan (T23A, R24A, Y26A)

This study

GL805D

GL504 A5-D1D3-sfgfp::kan (R27A, E28A, E29A)

This study

GL806D

GL504 A6-D1D3-sfgfp::kan (K33A, S34A)

This study

GL807D

GL504 A7-D1D3-sfgfp::kan (D42A, S43A, E44A, D45A)

This study

GL808D

GL504 A8-D1D3-sfgfp::kan (D49A, T52A, D53A, S54A)

This study

GL809D

GL504 A9-D1D3-sfgfp::kan (T60A, Q61A, S62A, Q64A)

This study

GL814D

GL504 A14-D1D3-sfgfp::kan (N327A, D329A)

This study

GL815D

GL504 A15-D1D3-sfgfp::kan (Q339A, Y340A, D343A)

This study

GL816D

GL504 A16-D1D3-sfgfp::kan (E346A, E347A)

This study

GL817D

GL504 A17-D1D3-sfgfp::kan (C352A, Q353A, Q354A)

This study

GL818D

GL504 A18-D1D3-sfgfp::kan (D363A, K366A)

This study

GL819D

GL504 A19-D1D3-sfgfp::kan (H370A, D374A, Q375A)

This study

GL820D

GL504 A20-D1D3-sfgfp::kan (E384A, K387A, R392A)

This study

GL821D

GL504 A21-D1D3-sfgfp::kan (S398A, R403A, D404A)

This study

GL822D

GL504 A22-D1D3-sfgfp::kan (K406A, K409A, Q410A)

This study

GL823D

GL504 A23-D1D3-sfgfp::kan (E416A, R419A)

This study

GL824D

GL504 A24-D1D3-sfgfp::kan (T426A, Y427A, T428A)

This study

GL825D

GL504 A25-D1D3-sfgfp::kan (Q429A, T430A, H431A, D433A)

This study

GL826D

GL504 A26-D1D3-sfgfp::kan (E437A, K440A, H441A)

This study

GL827D

GL504 A27-D1D3-sfgfp::kan (K443A, E444A, N445A, N448A)

This study

GL829D

GL504 A29-D1D3-sfgfp::kan (T453A, T455A, Y456A, E457A)

This study

GL831D

GL504 A31-D1D3-sfgfp::kan (R462A, H463A, T465A)

This study

GL832D

GL504 A32-D1D3-sfgfp::kan (K467A, K469A, E470A)

This study

GL833D

GL504 A21a-D1D3-sfgfp::kan (S398A)

This study

  1. a frt indicates the presence of the following oligonucleotide scar that was left after removing the kan cassette: GAAGTTCCTATACTTTCTAGAGAATAGGAACTTC. The “A##” numbers correspond to the site-directed mutations described in Tables 1 and 2.