BMC Microbiology

Table 4 Indole activity of selected disassembly-delay mutants of TnaA

From: A new suite of tnaA mutants suggests that Escherichia coli tryptophanase is regulated by intracellular sequestration and by occlusion of its active site

Variant ^a	Cells with foci (%)					Indole production (mM)
	2 h	3 h	4 h	6 h	12 h	2 h	3 h	4 h	6 h	12 h
Wild type	100	100	100	75	67	0.02	0.25	0.55	0.59	0.50
A2	41	100	100	100	64	0.00	0.04	0.04	0.13	0.05
A9	100	100	100	100	72	0.01	0.05	0.03	0.12	0.07
A17	100	100	100	100	100	0.02	0.06	0.03	0.16	0.15
A21a	100	100	100	100	87	0.01	0.06	0.24	0.43	0.45

^aThe number of foci and enzyme activity of specified mutants of TnaA-sfGFP fusion proteins.

Back to article page

ISSN: 1471-2180

Contact us

General enquiries: journalsubmissions@springernature.com