Table 3 Mutations that delay TnaA focus disassembly in stationary phase (Category 3)
Variant | Residue(s) changed to alanine | Residue location a | TnaA-sfGFP localization b | D1D3-sfGFP localization c | Indole d (%) |
|---|---|---|---|---|---|
A2 | E9, R12, R14 | D1D31-D1D32 interface | Delayed | Wild type | 7.2 |
A5 | R27, E28, E29 | D1 surface | Wild type | Wild type | 14.9 |
A7 | D42, S43, E44, D45 | D1 surface | Wild type | Wild type | 36.8 |
A8 | D49, T52, D53, S54 | D1D31-D24 | Wild type | Wild type | 6.6 |
A9 | T60, Q61, S62, Q64 | D1D31-D23 | Wild type | Diffuse | 5.3 |
A13 | K270 | D2 active site | Wild type | N/A | 10.9 |
A17 | C352, Q353, Q354 | D3 pocket | Wild type | Wild type | 6.0 |
A20 | E384, K387, R392 | D3 surface (EK) | Wild type | Wild type | 4.8 |
| Â | Â | D1D31-D24 (R) | Â | Â | Â |
A21 | S398, R403, D404 | Edge of the pocket | Ovoid | Wild type | 5.8 |
A21a | S398 | Bottom of the loop | Ovoid | Wild type | 43.4 |
A29 | T453, T455, Y456, E457 | D3 surface | Wild type | Wild type | 11.7 |