Variant | Residue(s) changed to alanine | Residue location a | TnaA-sfGFP localization b | D1D3-sfGFP Localization c | Indole d (%) |
---|---|---|---|---|---|
A2 | E9, R12, R14 | D1D31-D1D32 interface | Delayed | Wild type | 7.2 |
A24 | T426, Y427, T428 | D1D31-D1D32 interface | Delayed | Wild type | 8.5 |
A25 | Q429, T430, H431, D433 | D1D31-D1D32 interface | Delayed | Wild type | 13.3 |
A9 | T60, Q61, S62, Q64 | D1D31-D23 | Wild type | Diffuse | 5.3 |
A21 | S398, R403, D404 | Edge of the pocket | Ovoid | Wild type | 5.8 |
A21a | S398 | Bottom of the loop | Ovoid | Wild type | 43.4 |
A23 | E416, R419 | Edge of the pocket | Ovoid | Wild type | 5.1 |
A23a | E416 | Bottom of the loop | Ovoid | N/Ae | 12.1 |
A31 | R462, H463, T465 | Edge of the pocket | Ovoid | Wild type | 2.1 |
A31a | R462 | Bottom of the loop | Ovoid | N/A | 57.1 |