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Table 2 Mutations that alter TnaA focus formation during exponential growth (Category 2)

From: A new suite of tnaA mutants suggests that Escherichia coli tryptophanase is regulated by intracellular sequestration and by occlusion of its active site

Variant

Residue(s) changed to alanine

Residue location a

TnaA-sfGFP localization b

D1D3-sfGFP Localization c

Indole d (%)

A2

E9, R12, R14

D1D31-D1D32 interface

Delayed

Wild type

7.2

A24

T426, Y427, T428

D1D31-D1D32 interface

Delayed

Wild type

8.5

A25

Q429, T430, H431, D433

D1D31-D1D32 interface

Delayed

Wild type

13.3

A9

T60, Q61, S62, Q64

D1D31-D23

Wild type

Diffuse

5.3

A21

S398, R403, D404

Edge of the pocket

Ovoid

Wild type

5.8

A21a

S398

Bottom of the loop

Ovoid

Wild type

43.4

A23

E416, R419

Edge of the pocket

Ovoid

Wild type

5.1

A23a

E416

Bottom of the loop

Ovoid

N/Ae

12.1

A31

R462, H463, T465

Edge of the pocket

Ovoid

Wild type

2.1

A31a

R462

Bottom of the loop

Ovoid

N/A

57.1

  1. Legend is the same as for Table 1.