Strain
|
Ampicillin
|
Spectinomycin
|
---|
WT | 400 | 25 |
csrA
| 25 | <6.25 |
VC | 25 | <6.25 |
csrA/csrA+
| 200 | 25 |
- Strains GY123 (WT), GY448 (csrA), GY6536 (VC) and GY6535 (csrA/csrA+) were grown to 0.5 McFarland standard in TYE and inoculated into TYE broth containing concentrations of ampicillin or spectinomycin diluted 1:2 and ranging from 400 mg/ml to 6.25 mg/ml. MIC was determined based on visible growth in samples after incubation for 36 hours at 26°C. Results from three independent experiments performed in duplicate.