Figure 3
From: Mesoionic compounds with antifungal activity against Fusarium verticillioides
The effect of mesoionic compounds on ergosterol production by F. verticillioides . (A) Conidia were incubated at 26°C for 48 h in RPMI 1640 medium in the absence (a, control system) or presence of 3.9 μg/mL (c) or 7.8 μg/mL (d) of the mesoionic compounds MI-16. (B) Fungal cells not treated (a, control) or treated with 7.8 μg/mL (b) or 15.6 μg/mL (c) of MI-2 or with 3.9 μg/mL (d) or 7.8 μg/mL (e) of MI-11. After treatment, total lipids were extracted, and the obtained neutral lipids were applied to HPTLC plates. Ergosterol (ERG) and lanosterol (LAN) were used as sterol standards, as indicated by arrows. The use of 1% DMSO as eluent for the compounds did not alter the ergosterol levels (b, panel A). Densitometric quantifications (C and D) of the bands corresponding to panels A and B, respectively. Graphical representation of HPTLC data, which were analyzed using the Image J software. The sterol content of the control was set as 100%. Symbols denote significant differences (◈, P <0.05 Student’s t test) when compared to control cells (no treatment).