LpxA and RipA co-immunoprecipitate. The left two Western blots demonstrate LpxA-HA binds and pulls down RipA from F. tularensis whole cell lysates. The Western blots on the left were probed with primary antibodies to anti-HA and anti-N-terminal RipAaa1-19. The right two Western blots demonstrate that RipA-HA binds and pulls down LpxA-V5 from F. tularensis whole cell lystates. Western blots on the right were probed with primary antibodies to anti-HA and anti-V5. This figure is a representative of at least three independent experiments.