The S102 extragenic suppressor mutant phenotype and repair in J774A.1 and TC-1 cells. Intracellular replication of wild type F. tularensis LVS, ∆ripA, S102 (T36N), and the strain containing the repaired S102 extragenic suppressor mutation (LpxA N36 → T) was assessed within J774A.1 macrophages (A) and TC-1 epithelial cells (B) using the gentamicin protection assay. Each graph is the mean of at least three independent experiments done in triplicate, and the error bars represent the standard deviation. Statistical significance was determined using Student’s t tests comparing LpxA T36N to ∆ripA or to LpxA N36 → T36. *, P < 0.05.