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Table 3 Identification techniques used in CF clinical microbiology laboratories no. (%)

From: Microbiological diagnostic procedures for respiratory cystic fibrosis samples in Spain: towards standard of care practices

 

Bacteria

Mycobacteria

Yeasts

Filamentous fungi

BCT

17 (100)

9 (53)

8 (47)

9 (53)

Agglutination assays

17 (100)

-

-

-

SFT

-

-

11 (65)

-

Microscopy

-

-

-

15 (88)

MALDI-TOF

10 (59)

9 (53)

8 (47)

8 (47)

PCR + sequencing

    

rDNAa

11 (65)c

8 (47)d

6 (35)e

6 (35)f

ITS region

-

-

7 (41)e

8 (47)f

Othersb

5 (29)c

-

-

-

Hybridization assays

-

14 (82)

-

-

RFLPs

-

3 (18)d

-

-

  1. BCT: Biochemical tests; SFT: serum filamentation tests. RFLPs: Restriction Fragment Length Polymorphisms.
  2. a16S rDNA in the case of bacteria and 18S rDNA in the case of fungi.
  3. b recA gene, hsp65 gene, etc.
  4. c4 centers reported the use of >1 technique.
  5. d3 centers reported the use of both techniques.
  6. e, f4 centers reported the use of both techniques.