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Figure 3 | BMC Microbiology

Figure 3

From: Chlamydia pneumoniae effector chlamydial outer protein N sequesters fructose bisphosphate aldolase A, providing a benefit to bacterial growth

Figure 3

Changes in chlamydial infectious particle numbers in aldolase A knockdown cells. (A) Representative aldolase A protein expression images in knockdown HEp-2 cells. scRNA, scramble RNA transfected cells. ALDOA, aldolase A targeting siRNA transfected cells. (B) Representative images of inclusions formed in the knockdown HEp-2 cells. Chlamydiae infected knockdown cells at MOI 5, and were incubated for up to 3 days. Morphological changes in the inclusions were assessed under fluorescence microscopy at 3 (for C. pneumoniae) or 2 (for C. trachomatis) days after infection. Green, inclusions. (C, D) Changes in chlamydial infectious particle (IFU) numbers in the knockdown cells. Bacterial IFUs were evaluated by IFU assay. The IFU numbers were also assessed at the same time as above. Data show mean ± SD obtained from at least three independent experiments. *p < 0.05 versus value into scRNA-transfected cells. N.S., not significant. C. pneumoniae (C). C. trachomatis (D).

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BMC Microbiology

ISSN: 1471-2180

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