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Figure 1 | BMC Microbiology

Figure 1

From: Chlamydia pneumoniae effector chlamydial outer protein N sequesters fructose bisphosphate aldolase A, providing a benefit to bacterial growth

Figure 1

Sequence similarity between C. pneumoniae and C. trachomatis CopNs and representative pull-down images. (A) Sequence similarity between C. pneumoniae and C. trachomatis CopNs. Amino acid sequence data were obtained from NCBI genome site (http://www.ncbi.nlm.nih.gov/genome/). C. pneumoniae TW183 CopN, CpB0334 (accession number: NC_005043.1). C. trachomatis UW-3/CX serovar D CopN, CT_089 (accession number: NC_000117.1). Alignment was constructed by web software, UniProt (http://www.uniprot.org/). C. pneumoniae CopN had 48% homology as compared with C. trachomatis CopN. Stars highlighted gray indicate identical amino acids. (B) Protein expression profiles of constructed recombinant GST-fused CopNs. Cpn, C. pneumoniae CopN full length (399 aa) (GST-CpCopN). CtD, C. trachomatis CopN full length (421 aa) (GST-CtCopN). GST, GST alone. (C) Representative pull-down images with GST-fused chlamydial CopNs in HEp-2 cell lysates. Red arrow shows a specific band captured by GST–CpCopN, but not GST–CtCopN, in the HEp-2 cell lysates.

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BMC Microbiology

ISSN: 1471-2180

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