|
V. cholerae
strains
| | |
|
A1552
|
Wild-type, O1 El Tor Inaba, RifR
|
[52]
|
|
A1552-LacZ-Kan
|
A1552 strain with aph cassette in lacZ gene; RifR, KanR
|
[53],[54]
|
|
A1552-TntfoX
|
A1552 containing mini-Tn7-araC-P
BAD
-tfoX; RifR, GentR
|
[10]
|
|
ΔhapR
|
A1552ΔVC0583, RifR
|
[4]
|
|
ΔhapR-TntfoX
|
A1552ΔhapR containing mini-Tn7-araC-P
BAD
-tfoX; RifR, GentR
|
[10]
|
|
ΔcomEA
|
A1552ΔVC1917 (=A1552VC1917 in (Ref)), RifR
|
[4]
|
|
ΔcomEA-TntfoX
|
A1552ΔcomEA containing mini-Tn7-araC-P
BAD
-tfoX; RifR, GentR
|
[22]
|
|
ΔqstR
|
A1552ΔVC0396, RifR
|
[22]
|
|
ΔqstR-TntfoX
|
A1552ΔqstR containing mini-Tn7-araC-P
BAD
-tfoX; RifR, GentR
|
[22]
|
|
ΔCRP-S
|
CRP-S site upstream of comEA deleted in strain A1552-TntfoX using the TransFLP method; RifR, GentR
|
This study
|
|
CRP-S_inv
|
CRP-S site upstream of comEA inverted in strain A1552-TntfoX using the TransFLP method; RifR, GentR
|
This study
|
|
CRP-N
|
CRP-S site upstream of comEA changed for a CRP-N site (see scheme in Figure 4) in strain A1552-TntfoX using the TransFLP method; RifR, GentR
|
This study
|
|
[frdA] site
|
CRP-S site upstream of comEA changed for the in silico predicted CRP-N site preceding the frdA gene in strain A1552-TntfoX (see scheme in Figure 4) using the TransFLP method; RifR, GentR
|
This study
|
|
CRP-0
|
CRP-S site upstream of comEA changed in the 3′ conserved region (see scheme in Figure 4) in strain A1552-TntfoX using the TransFLP method; RifR, GentR
|
This study
|
|
WT_qstR (FRT control)
|
Extended TransFLP scar [53],[55] added upstream of qstR without changing the CRP-S site (control) in strain A1552-TntfoX; RifR, GentR
|
This study
|
|
ΔHapR-site_qstR
|
HapR-binding site determined in vitro[22] deleted from strain A1552-TntfoX using the TransFLP method; RifR, GentR
|
This study
|
|
ΔCRP-S_qstR
|
CRP-S site upstream of qstR deleted in strain A1552-TntfoX (see scheme in Figure 4) using the TransFLP method; RifR, GentR
|
This study
|
|
[frdA] site_qstR
|
CRP-S site upstream of qstR changed for the in silico predicted CRP-N site preceding the frdA gene (see scheme in Figure 4) in strain A1552-TntfoX using the TransFLP method; RifR, GentR
|
This study
|
|
CRP-0_qstR
|
CRP-S site upstream of qstR changed in the 3′ conserved region (see scheme in Figure 4) in strain A1552-TntfoX using the TransFLP method; RifR, GentR
|
This study
|
|
Plasmids
| | |
|
pBR322
|
AmpR, TcR
|
[56]
|
|
pBAD/Myc-HisA
|
pBR322-derived expression vector; araBAD promoter (PBAD); AmpR
|
Invitrogen
|
|
p_qstR
|
qstR gene cloned into pBAD/Myc-HisA; arabinose inducible; AmpR
|
[22]
|
|
pUX-BF13
|
ori R6K, helper plasmid with Tn7 transposition function; AmpR
|
[57]
|
|
pGP704::Tn7
|
pGP704 with mini-Tn7
|
[58]
|
|
pGP704-mTn7-araC-tfoX
|
pGP704 with mini-Tn7 carrying araC and P
BAD
-driven tfoX; AmpR
|
[10]
|
|
pBR-Tet_MCSI
|
pBR322 derivative deleted for Tet promoter and part of tet
R gene; new MCS included; AmpR
|
[10]
|
|
pBR-Tet_MCSII
|
pBR322 derivative deleted for Tet promoter and part of tet
R gene; new MCS included; AmpR
|
[22]
|
|
pBR-[own]comEA
|
comEA gene preceded by 900 bp of upstream region cloned into pBR-Tet_MCSII; AmpR
|
[22]
|
|
pBR-[−700]comEA
|
comEA gene preceded by 700 bp of upstream region; plasmid generated by inverse PCR of pBR-[own]comEA; AmpR
|
This study
|
|
pBR-[−500]comEA
|
comEA gene preceded by 500 bp of upstream region; plasmid generated by inverse PCR of pBR-[own]comEA; AmpR
|
This study
|
|
pBR-[−300]comEA
|
comEA gene preceded by 300 bp of upstream region; plasmid generated by inverse PCR of pBR-[own]comEA; AmpR
|
This study
|
|
pBR-[−134]comEA
|
comEA gene preceded by 134 bp of upstream region cloned into Not I site of pBR-Tet_MCSII; AmpR
|
This study
|
|
pBR-[−100]comEA
|
comEA gene preceded by 100 bp of upstream region; plasmid generated by inverse PCR of pBR-[own]comEA; AmpR
|
This study
|
|
pBR-[−40]comEA
|
comEA gene preceded by 40 bp of upstream region; plasmid generated by inverse PCR of pBR-[own]comEA; AmpR
|
This study
|
