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Table 1 Strains and plasmids used in this study

From: Regulatory elements involved in the expression of competence genes in naturally transformable Vibrio cholerae

Strains or plasmids Genotype*/Description Reference
V. cholerae strains   
A1552 Wild-type, O1 El Tor Inaba, RifR [52]
A1552-LacZ-Kan A1552 strain with aph cassette in lacZ gene; RifR, KanR [53],[54]
A1552-TntfoX A1552 containing mini-Tn7-araC-P BAD -tfoX; RifR, GentR [10]
ΔhapR A1552ΔVC0583, RifR [4]
ΔhapR-TntfoX A1552ΔhapR containing mini-Tn7-araC-P BAD -tfoX; RifR, GentR [10]
ΔcomEA A1552ΔVC1917 (=A1552VC1917 in (Ref)), RifR [4]
ΔcomEA-TntfoX A1552ΔcomEA containing mini-Tn7-araC-P BAD -tfoX; RifR, GentR [22]
ΔqstR A1552ΔVC0396, RifR [22]
ΔqstR-TntfoX A1552ΔqstR containing mini-Tn7-araC-P BAD -tfoX; RifR, GentR [22]
ΔCRP-S CRP-S site upstream of comEA deleted in strain A1552-TntfoX using the TransFLP method; RifR, GentR This study
CRP-S_inv CRP-S site upstream of comEA inverted in strain A1552-TntfoX using the TransFLP method; RifR, GentR This study
CRP-N CRP-S site upstream of comEA changed for a CRP-N site (see scheme in Figure 4) in strain A1552-TntfoX using the TransFLP method; RifR, GentR This study
[frdA] site CRP-S site upstream of comEA changed for the in silico predicted CRP-N site preceding the frdA gene in strain A1552-TntfoX (see scheme in Figure 4) using the TransFLP method; RifR, GentR This study
CRP-0 CRP-S site upstream of comEA changed in the 3′ conserved region (see scheme in Figure 4) in strain A1552-TntfoX using the TransFLP method; RifR, GentR This study
WT_qstR (FRT control) Extended TransFLP scar [53],[55] added upstream of qstR without changing the CRP-S site (control) in strain A1552-TntfoX; RifR, GentR This study
ΔHapR-site_qstR HapR-binding site determined in vitro[22] deleted from strain A1552-TntfoX using the TransFLP method; RifR, GentR This study
ΔCRP-S_qstR CRP-S site upstream of qstR deleted in strain A1552-TntfoX (see scheme in Figure 4) using the TransFLP method; RifR, GentR This study
[frdA] site_qstR CRP-S site upstream of qstR changed for the in silico predicted CRP-N site preceding the frdA gene (see scheme in Figure 4) in strain A1552-TntfoX using the TransFLP method; RifR, GentR This study
CRP-0_qstR CRP-S site upstream of qstR changed in the 3′ conserved region (see scheme in Figure 4) in strain A1552-TntfoX using the TransFLP method; RifR, GentR This study
Plasmids   
pBR322 AmpR, TcR [56]
pBAD/Myc-HisA pBR322-derived expression vector; araBAD promoter (PBAD); AmpR Invitrogen
p_qstR qstR gene cloned into pBAD/Myc-HisA; arabinose inducible; AmpR [22]
pUX-BF13 ori R6K, helper plasmid with Tn7 transposition function; AmpR [57]
pGP704::Tn7 pGP704 with mini-Tn7 [58]
pGP704-mTn7-araC-tfoX pGP704 with mini-Tn7 carrying araC and P BAD -driven tfoX; AmpR [10]
pBR-Tet_MCSI pBR322 derivative deleted for Tet promoter and part of tet R gene; new MCS included; AmpR [10]
pBR-Tet_MCSII pBR322 derivative deleted for Tet promoter and part of tet R gene; new MCS included; AmpR [22]
pBR-[own]comEA comEA gene preceded by 900 bp of upstream region cloned into pBR-Tet_MCSII; AmpR [22]
pBR-[−700]comEA comEA gene preceded by 700 bp of upstream region; plasmid generated by inverse PCR of pBR-[own]comEA; AmpR This study
pBR-[−500]comEA comEA gene preceded by 500 bp of upstream region; plasmid generated by inverse PCR of pBR-[own]comEA; AmpR This study
pBR-[−300]comEA comEA gene preceded by 300 bp of upstream region; plasmid generated by inverse PCR of pBR-[own]comEA; AmpR This study
pBR-[−134]comEA comEA gene preceded by 134 bp of upstream region cloned into Not I site of pBR-Tet_MCSII; AmpR This study
pBR-[−100]comEA comEA gene preceded by 100 bp of upstream region; plasmid generated by inverse PCR of pBR-[own]comEA; AmpR This study
pBR-[−40]comEA comEA gene preceded by 40 bp of upstream region; plasmid generated by inverse PCR of pBR-[own]comEA; AmpR This study
  1. *VC numbers according to [59].
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